Botulinum toxins are tested on small Swiss mice. This is the bases for measuring the strength of the product. The FDA requires that each lots undergo this type of assay to validate its strength. That is true of any botulinum toxin use for human being. Please be aware that clever scammers are out there to cash in of the botox gravy train by pushing products with confusing names that sound like they are doing something like botox. These products are not botox and they are not effective. They simply separate you from your money.
Thank you for asking this question. Unfortunately on the market right now, there are no vegan alternatives to Botox. All Neuromodulators (Botox, Xeomin, Dysport, etc..) are tested on animals which is a required process for FDA approval. However, it does not mean that in future, research could not be done in this area, to provide an alternative.