Is there a maximum amount of fat that can be injected? What is the maximum volume of the fat that can be harvested?

The limit to the amount of fat to be grafted is either the paucity of donor fat or the size of the recipient area.  Although ideal fat survival depends on many things including meticulous harvest, process and injection technique...perhaps the most important thing is not to over inject.  Fat must be surrounded by healthy tissue to provide nutrients and blood flow.  Essentially you can double the size of a breast or buttock at the time of surgery.  Assuming that 50-60% of the volume injected remains, then you will get a breast of buttock that is 50-60% larger than it started.

I typically transfer from 250 cc to 400 cc or so per breast. Fat is placed within the breast, not in the muscle.  About 50% of the fat obtains permanence.
If you are comfortable with about a one cup size increase, fat transfer would be reasonable. If you want 2 or more cups, breast augmentation with implants would be better.  I typically reserve fat grafting for very small augmentations, particularly in women who have failed breast implants or who have been reconstructed.Kenneth Hughes, MD, Board Certified Plastic SurgeonLos Angeles, CA

There is not a specific limit. However, transferred fat should be surrounded by tissue with good blood supply. If the fat is not surrounded by tissue with good blood supply it is not going to survive. The amount of fat that could be transferred per breast will vary based on your breast size. Good luck!