This is an excellent question Wendy. Botox treatments are best done 2 or more weeks before CO2. Botox reaches its full effectiveness at 2 weeks after injection; your CO2 results will benefit from decreased facial muscle movement while healing from the CO2. Botox injected directly before CO2 could be affected by swelling associated with the CO2 treatment. Swelling may cause the botox to diffuse away from the intended muscles and toward unintended muscles. This could result in a heavy brow, heavy lid, or less effective result on the desired areas. Botox is prepared for injection by dissolving it into saline. When injected, the saline spreads into the tissues taking the botox (a small protein) with it. The botox protein then moves from the tissue space into the muscle cells, where it becomes active in relaxing the muscle. The botox product monograph recommends staying upright for 4 hours following administration. Based on clinical experience, this is likely an exaggerated recommendation. I continue to recommend waiting the four hours to ensure the botox is completely absorbed in the muscle prior to performing CO2 treatment. The CO2 laser treatment should not cause any harm to the botox protein. Additionally, CO2 will not have a negative effect on dermal filler. Filler injections are best done a few weeks prior to CO2 in order to allow both the client and physician to monitor the skin carefully for any potential vascular complications from the filler. Having your botox treatment immediately after CO2 is less desirable because application of your post treatment products and swelling from the CO2 might result in botox diffusion. Dermal filler immediately after CO2 is not recommended. Best results will follow a comprehensive plan that incorporates medical grade skin care, botox, and dermal filler treatments well before any CO2 treatments.