Had botox for my frown lines. I pushed my eyebrow arch up with my finger to adjust my brow hairs. Will it affect my result?

This is very unlikely to negatively affect your results. Time will tell, however. You should know for sure 5-7 days post Botox treatment. I would not worry about this however since it is very unlikely that you have caused a problem. I hope this information is helpful for you.Stephen Weber MD, FACSDenver Facial Plastic Surgeon

Accidentally poking or scratching of the brow after Botox injection 45 minutes after the procedure is very unlikely to cause any trouble.  We recommend avoiding massaging or rubbing the area for the first few hours after the procedure, but a few "pokes" should have minimal effect.  The full effect of the Botox will take up to 7 days to appreciate in most cases.

Given that you had pushed the eyebrow arch 45 minutes after the procedure the likelihood of negative effects as a result of your "motion" is minimal. It would be a greater concern if you were to do this immediately after procedure.