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Botox is not required to in order to put the transplanted onto the forehead area. However, starting the hairline right on the forehead might probably result in unnatural hairline since it's too low. Hair loss is a continuous process and it's better to save limited number of donor hair for future hair loss too.
frontal hairline should be designed according to facial anatomy and actions of frontalis muscle. Botox injections to frontalis muscle in order to plan and have a lower frontal hairline is non sense and not logical .