Latisse is applied daily with a disposable brush which is included with product. Latisse is gently applied along the base of the upper eyelashes. After three or four months, Latisse can be used much less frequently to maintain longer, thicker lashes. There are approximately 100 to 150 eyelashes on each upper eyelid. The growth of eyelashes is cyclical, going through 3 stages: a growth phase, a transition phase, and a resting phase. At any given time, numerous hair follicles can be found in each of the different stages. The growth phase of an average eyelash hair is approximately 1 to 2 months. Latisse has been shown to increase the amount of time each eyelash hair remains in the growing phase. The application of Latisse has also been scientifically proven to increase the thickness of eyelash hair as well as the darkness of each hair. This is most likely due to an increased production of pigment within each hair follicle. Typically, within 4 months of application, Latisse can increase the length, thickness, and darkness of eyelashes and improve all the traits that are associated with greater prominence and overall attractiveness of the eyelashes.