No , masturbation does not cause hair loss. The reason of the hair loss may related with factors other than genetic like connective tissue disorders, stress, anemia, lupus, medications, hormonal and seasonal changes, nutritional problems, severe diets, bulimia, protein/calorie deficiency, zinc and essential amino-acid deficiency, mal-absorption (intestinal and digestive problems), A-vitamin excess, general anesthesia, affective mood disorders. Drugs that may cause hair loss in woman Anti-coagulants such as warfarin and heparin Epilepsy drugs, especially dilantinGout drugs, allopurinol and colchicumAntihypertensive drugs, diuretics, beta blockers.... etcSo you may need dermatoscopy and ıf needed a skin biopsy and to have a full exam with an hair surgeon and/or endocrinologist , to find out any reason for hair loss.