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Botox is mixed with 2.5 cc saline per 100 unit bottle usually now but some docs will use 1 cc and some use 3 -4 cc. Whatever the concentration that they mix up, they know the units they are injecting to each area so trust them. Botox can be even further diluted when you are getting it for sweating palms, feet and under arms. Don't try to inject Botox if you are not a doc or RN. There are definite techniques we use for different effects and areas.
Whether the physician dilutes the Botox powder with a diluent of 1, 2, 2.5 or 4 ccs of saline, the important variable which determines your result, is the total number of units given. If doctor B uses a 2cc dilution they will have to give twice the volume of diluted Botox to create the same effect as doctor A who uses a 1 cc dilution. Both doctors will have administered the same number of units! If the dilution is excessive, then the larger volume might make the Botox travel via gravity or spread of fluid to surrounding areas and there might be an affect on muscles that were not targeted.
Allergan recommends mixing 2.5 cc of saline per 100 units of Botox.There is a wide range of what doctors use--anywhere from 1 to 4 cc per 100 units. I think what is more important in achieving the results you desire isSkill of injector--who is injecting you?Make sure using real BotoxMake sure it isn't watered downMake sure fresh BotoxGood luck!
Typically, botox is diluted from 1 to 4cc of saline depending upon the injector and his or her experience. In my opinion, the most important consideration is how many units are you receiving for a particular area. Inadequate units are the most common reason for a less than desired effect while too many units or injection in the wrong place can cause side effects like eyelid or eyebrow drooping.
I would refer to the package insert when it comes to preparing and injecting Botox for administration.There are many marks on the insulin syringe and if you are referring to the o.1ml mark than diluting it with 1cc of saline.I hope that you are a medical professional asking this question with the proper credentialing to prepare this.