Hello and thank you for your question! It is always a good idea to double check before combining any treatments. In short, no, derma rolling won’t reverse the effects of Botox once it’s fully settled (typically after 10–14 days). But here’s the nuance: If you derma roll too soon after Botox—within the first few days—there’s a small risk of moving the Botox from where it was injected. This could reduce its effectiveness or cause it to spread to unintended areas, leading to uneven results. Once Botox has bound to the muscle, which usually happens within 2 weeks, it’s very unlikely that rolling or microneedling will interfere with it.So, it’s not that derma rolling “reverses” Botox, but if done too early, it could interfere with how well it works. To be safe, wait at least 10–14 days after your injections before derma rolling, use gentle pressure, and avoid areas that were heavily treated if you’re unsure. If you’re ever in doubt, your injector can confirm exactly when it’s okay for your specific treatment. I hope this helped! Genie Alexander, RN BSN for Anna Petropoulos, MD FRCS Center for Classic Beauty Danvers & Boston, MA