Is Botox Mixed with Saline or Sterile Water and Once Mixed, How Long Can It Be Stored For?

Is botox mixed with saline or sterile water and once mixed, how long can it be stored for?

Doctor Answers 18

Botox done by experienced doctors

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The storage of a typical bottle of Botox may only be a few days in busy offices. Most doctors use an off-label mixture of saline with preservative to mix their Botox and store it in the refrigerator for up to two weeks if needed, some may hold it longer but that again, is off-label.

Manhattan Dermatologic Surgeon
4.9 out of 5 stars 39 reviews

Botox mixing

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Botox is typically reconstituted with normal saline (with or without preservative)  and can last for likely up to 6 weeks (some think even longer) when refrigerated. Most busy and successful practices will use up their Botox within 1-3 days.

Benjamin Barankin, MD, FRCPC
Toronto Dermatologic Surgeon

Mixing and storage of Botox

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Botox is mixed with normal saline (bacteriostatic) and can last for several weeks under proper refrigeration.  In most practices, it is used up the same day or within a few days.

Matheson A. Harris, MD
Salt Lake City Oculoplastic Surgeon
5.0 out of 5 stars 6 reviews

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Botox is mixed with bacteriostatic saline

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Interesting question and am unsure exactly why you are asking, but Botox is mixed with bacteriostatic saline. It is then refrigerated and can be kept there for a few weeks. However, in high volume offices, like mine, I use several vials a day, so the most it sits in there is a few hours.

Studies have shown Botox is stable forup to 6 weeks once reconstituted

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Numerous studies have shown that the Botox molecule is stable, even with agitation, for at least 6 weeks after being reconstituted and refrigerated.  I have provided a link below of a recent article confirming these observations.

Curt Samlaska, MD
Las Vegas Dermatologist

Preparation of Botox

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Frankly, I am curious to know why you are asking this question. But to answer you, Botox is reconstituted with sterile saline and can last several weeks in the refrigerator after being mixed. However, in a a busy Botox practice, several vials are used daily so that Botox doesn't sit around mixed for more than a day usually.

Channing R. Barnett, MD
Boca Raton Dermatologist
4.1 out of 5 stars 8 reviews

Mix Botox with Saline

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Sterile saline (salt water) is the best liquid for mixing Botox. Although it does last many days reconstituted, many think is slowly loses its potency once mixed.

Many offices like to mix it and use it immediately. We agree with this.

Christopher J. Peers, MD
South Bend Facial Plastic Surgeon

Botox preparation and storage

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Botox and other Botulotoxin preparations are normally reconstituted with bacteriostatic normal saline to reduce stinging sensation during injections.

Once reconstituted it should be used up. However, it is believed its efficacy can last from 1-6 weeks if stored in a fridge.

Botox and duration of effectiveness

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Botox is reconstituted with sterile saline and can last many weeks in the refrigerator after being mixed.  In a busy injector's office, a bottle lasts less than a day. 

Martie Gidon, MD, FRCPC
Toronto Dermatologist

Botox reconstitution and storage

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Botox is reconstituted with normal saline and is then refrigerated in a medical refrigerator. It can stay for several weeks under these conditions and still be effective. However, in practices that treat patients with Botox regularly, it isn't around for more than a day or two so this does not become a concern.

These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.