Is Botox Mixed with Saline or Sterile Water and Once Mixed, How Long Can It Be Stored For?

The storage of a typical bottle of Botox may only be a few days in busy offices. Most doctors use an off-label mixture of saline with preservative to mix their Botox and store it in the refrigerator for up to two weeks if needed, some may hold it longer but that again, is off-label.

Botox is mixed with saline. It hurts ALOT less than mixing it with water. It is stable in the refigerator for a couple of weeks. If you are treated at an office that does alot of Botox injections, it is unlikely that the product sits in the refrigerator for more than a day. We use multiple vials per day and the longest the product sits in the refrigerator is about 24 hours. With that said, the product is stable for much longer than that if stored properly. I hope this information is helpful.Stephen Weber MD, FACS

Botox is typically reconstituted with normal saline (with or without preservative)  and can last for likely up to 6 weeks (some think even longer) when refrigerated. Most busy and successful practices will use up their Botox within 1-3 days.

Botox is mixed with normal saline (bacteriostatic) and can last for several weeks under proper refrigeration.  In most practices, it is used up the same day or within a few days.

Numerous studies have shown that the Botox molecule is stable, even with agitation, for at least 6 weeks after being reconstituted and refrigerated.  I have provided a link below of a recent article confirming these observations.