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Latisse is dispensed with 60 sterile brushes because the FDA regulations state it must be so. The tip of the dispensing bottle should never touch anything and be aware that the brushes are being applied to the eyes. It is for your safety that these instructions are given.
The reuse of eyelid applicators for Latisse is not indicated as an infection could ensue. It is not uncommon to see eyelid infections known as blepharitis. Reusing applicators, makeup brushes, mascara brushes may spread infections so it is important to follow the manufacturer's instructions.
Allergan, the producer of Latisse, recommends that applicators be used once for each eyelid. This is done to prevent liability for potential infection spread by multiple use of the applicators. If you decide to use the applicators more than once you would hardly be the only one doing so BUT you would be clearly operating outside of FDA and the company's recommendation.
If there are no signs of inflammation or infection in either eye, I recommend using one applicator for both eyes and then throwing the applicator away. You will waste less Latisse that way. If there are any signs of inflammation or infection you should not be using Latisse anyway until it has resolved.