Is my Eye Twitching Caused by Botox?

Botox is actually a treatment for eye twitching.  If you had an injection yesterday, the Botox will not even be active yet.  You may just have a minor irritation from the injection, but not anything due specifically from the Botox.  If you continue to have a problem, then you should discuss with your surgeon.

Probably, your twitching is related to separate factors than the Botox you had injected. Clinically, the number one factor I have seen in patients who have never had Botox but develop twitching in the eyelid is fatique.  The twitching can last for several days or more and then goes away.  Botox can inhibit the muscle contraction of a twitching area but whether your botox injection in an adjoining area caused the area not injected to be stimulated and then caused twitching will not be able to be determined.  If there had been many of these situations reported over the last two decades of Botox use then even if the pathophysiology were not understood, there would be a statistic to quote of its prevalence. For now, it would be important for you to inform your doctor and they can report it to Allergan, who can report it to the FDA. 

Botox helps correct twitching so it is not likely related to it.  Isolated eyelid twitching is called myokymia and is related to things like stress, caffeine, and not getting enough sleep.