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In order to get FDA clearance, a product has to prove to be effective and safe, and for stimulating eyelashes to grow fuller longer and darker, Latisse is the only one. Glaucoma drugs of any type - even Lumigan, which contains the same drug as Latisse - should not be used for eyelashes.
Timolol is an entirely different class of medications with a different mechanism. Thus it will not provide the expected aesthetic benefit that Latisse provides.
Latisse is FDA approved for eyelash growth, not Timoptic. My recommendation is to stay safe and use the effective dose and formulation! for eyelash growth that is in Latisse.
Timoptic is a completely different glaucoma drop and has never had any affect on lash growth. Latisse (Lumigan or Bimatoprost) is an effective enhancer of lashes, both as used cosmetically as Latisse on a brush as well as the glaucoma drop.
Timolol is a completely different medication and will not have the same effect on eyelash growth.