I have dispensed over 3000 Latisse/Lumigan bottles and in my experience permanent makeup fs safe to do while using Latisse, and visa versa. I personally have permanent eyeliner and have also been using Latisse (and previously Lumigan) for three years. Not only is it safe, but I have found that my permanent eyeliner has not needed to be touched up at all during these last three years, while previously it had lightened and needed touching up every two years. While no studies have been done looking at this, to my knowledge, I am guessing that the skin darkening "side effects" of bimatoprost, the active ingredient of Latisse, are also keeping the permanent makeup tattoo pigment or the skin around it dark. I am not sure of the mechanism for this but I am happy about this unexpected "side effect." I would recommend not using Latisse on the newly tattooed area for at least 1 week after tattooing, to avoid irritation.