Latisse is meant to be dispensed as a one month supply, with enough liquid and disposable applicators to last for 30 days. However, many patients are finding that it does last longer, sometimes for 6-8 weeks when using just one drop per upper eyelash per night (the recommended dose). The trick to extending its use (which is something the company is not allowed to tell you) is to use the same disposable applicator for both upper eyelids at night rather than a separate one for each eyelid. It comes with 60 applicators, so this way you can get 60 nights of application as long as the solution in the bottle lasts (usually 6-8 weeks). Definitely stick with the appropriate amount of 1 drop per upper eyelash per night though to get the best effects.