Does a vial of Botox come pre-mixed?

I have heard Botox has to be mixed each time it is used. Is this true, or does it come pre-mixed? Thanks.

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14 answers to “Does a vial of Botox come pre-mixed?”

A: Botox does not come pre-mixed

Steven E. Rasmussen, MD

As answered by my colleagues before me, Botox comes as dried powder that must be kept refrigerated. The vial contains either 50 or 100 units. It is reconstituted in normal saline prior to your treatment. There is no set volume of saline that is used. It is up to the individual provider. It ranges... more

A: Botox does not come pre mixed

Darrick E. Antell, MD

Botox is stored in a cold environment and has no liquid in it when the manufacturer delivers it. We generally use a minimal dilution as I think more concentrated Botox allows us to use fewer needle sticks.  Others dilute it more, but my concern is that higher dilutions may not last as long.

A: Botox is packaged as a dried product.

Bryan K. Chen, MD

Botox does not come pre-mixed.  It only needs to be mixed one time with sterile saline.  If possible, Botox should be mixed just prior to injection.

A: No, it does not come premixed in liquid.

Charles Crutchfield, MD

Botox Cosmetic does NOT come premixed in liquid.  It comes as an extremely small amount of powder in a vial that is 'lyophilized', which is a fancy term for 'freeze dried".  Botox cosmetic is measured in units.  Units are a way of measuring the acrtivity, so all vials will have the same... more

A: Mixed one time

Hisham Seify, MD, PhD

Botox Vial is 100 Units. It is mixed with saline with no preservative. The company recommendation is adding 2.5cm to the vial. Once it is mixed it could be used within a certain time limit.

A: Botox needs to be reconstituted

David Shafer, MD

Apeeling One, Botox come freeze-dried and needs to be reconstituted. It come in 100 unit and 50 unit vials. It needs to be mixed with preservative-free normal saline. The amount of saline used determines the concentration. I hope this is helpful. David Shafer, MD New York City

A: It does not come pre-mixed

David L. Kaufman, MD

Botox cosmetic comes as a purified protein - not in suspension.  It is reconstituted with some amount of saline - depending on the provider.  After it is reconstitued, it should be used within 4 hours - according to the most stringent recommendations.  Studies have shown, and it is our... more

A: Botox does not come pre-mixed.

Sanjay Grover, MD

Botox does not come pre-mixed and needs to be reconstituted with normal saline prior to injection. Once mixed, the effectiveness of Botox can last for a certain period of time.   Everyone dilutes their Botox with different amounts of normal saline, thus you should know how many 'units' of Botox that... more

A: Botox--Injector Picks Dilution

Anil R. Shah, MD

Botox does not come premixed. It comes freeze dried (kinda like astronaut food). The injector will help pick the dilution of botox depending on the intended application. Once botox is mixed it should ideally be used within 4 hours of reconstitution but this number is controversial. Botox can be made more... more

A: Each bottle needs to be mixed by the doctor's office

Richard P. Rand, MD

Botox comes from the manufacturer as a dry powder in a small vial. It needs to be reconstituted to its active form by the office using it. The concentration can vary depending on how much saline is added to the 100 unit vial.

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