I asked my mother who worked in Dr. Schantz's lab at Fort Detrick, where Botulinum toxin was developed, this question. She confirmed that they used mice in their experimentation in 1944. ( Unlike the Japanese who were using human prisoners for their testing.)
Allergan still employs what is called the Lethal Dose 50 percent (LD50) to assess the potency of Botox. This is performed on mice. There has been a concerted effort on the part of the Humane Society to have Allergan find anothe...