How to dilute a Botox vial

Sirish Maddali, MD answers: How much solution should be added per Botox vial?

If there are 1000 units of Botox in a vial, how much solution should be added?


Sirish Maddali, MD
2 months ago

As others have mentioned, Botox in the US comes in 100 U and 50 U vials. The 100 U one is the most common. Different providers dilute it in different ways. Personally, I use 2cc of saline. More important than the dilution is the number of units injected in a given area. HOWEVER, if too much fluid is injected in one area, then diffusion is greater and nearby muscles can be affected more than if a smaller amount of fluid is used. Therefore, in my experience, if 4 cc of saline is used for dilution and the frown lines between the brows are treated, there is a higher likelihood of botox migrating to the eyelid muscle, which can cause drooping. I hope this helps you. 

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